Journal: Cells

Article Title: Annexin A1/Formyl Peptide Receptor Pathway Controls Uterine Receptivity to the Blastocyst

doi: 10.3390/cells9051188

Figure Lengend Snippet: AnxA1 controlled Muc-1 expression on uterine epithelial cells via FPR1/FPR2, independent of IL-6 secretion. Muc-1 expression on uterine epithelial cells was determined 24 h after incubations ( A , B ). Muc-1 expression was determined 24 h after IL-6 treatment ( C ). IL-6 secretion was determined in the supernatant of uterine epithelial cells 24 h after treatments ( D ). (−) means absence and (+) means presence of treatments. The data are expressed as mean ± standard error of mean of three to five independent experiments. a p < 0.05 vs. NT; b,c p < 0.05 vs. AnxA1.

Article Snippet: In order to analyze FPR1, FPR2, CD61 and NF-kB expression, the cells were washed twice in PBS/BSA and incubated with specific antibodies as follows: FPR1-PE (1:100; #FAB3744P BD Biosciences, Minneapolis, MN, USA), FPR2-FITC (1:100; #bs3654R FITC; Bioss, Boston, MA, USA), CD61-FITC (1:50; #555753; BD Biosciences) or NF-kB (1:100; #0465R, Biolegend, San Diego, CA, USA) for 40 min in the dark at RT.

Techniques: Expressing

Journal: Cells

Article Title: Annexin A1/Formyl Peptide Receptor Pathway Controls Uterine Receptivity to the Blastocyst

doi: 10.3390/cells9051188

Figure Lengend Snippet: AnxA1 controlled claudin-1 and ZO-1 expressions on uterine epithelial cells via FPR1 and FPR2. Claudin-1 ( A ) and ZO-1 ( B ) expressions on uterine epithelial cells were determined 24 h after incubations. Representative images of claudin-1 and ZO-1 immunofluorescence are shown. (−) means absence and (+) means presence of treatments. The data are expressed as mean ± standard error of mean of three to five independent experiments. a p < 0.05 vs. NT; b p < 0.01 and c p < 0.05 vs. AnxA1.

Article Snippet: In order to analyze FPR1, FPR2, CD61 and NF-kB expression, the cells were washed twice in PBS/BSA and incubated with specific antibodies as follows: FPR1-PE (1:100; #FAB3744P BD Biosciences, Minneapolis, MN, USA), FPR2-FITC (1:100; #bs3654R FITC; Bioss, Boston, MA, USA), CD61-FITC (1:50; #555753; BD Biosciences) or NF-kB (1:100; #0465R, Biolegend, San Diego, CA, USA) for 40 min in the dark at RT.

Techniques: Immunofluorescence